The need of microbiological investigation and the procedure is explained to the patient.
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The availability of sterile swab sticks is checked.
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Two test tube are labeled with the patient’s name, date, and registration number.
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A requisition form with patient’s details, any comorbidities and ongoing treatment and site is filled.
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Hands are washed thoroughly.
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The wound is cleaned with normal saline before collection of sample.
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Use Sterile swabs with cotton tips.
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If the wound is moist swab is used straight from the packaging.
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If the wound is dry, the swab tip is moistened with normal saline to increase the chances of recovering organisms from the site.
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It is ensured that the swab only comes into contact with the wound surface.
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The swab is moved across the wound surface in a zigzag motion.
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A representative area of the wound is sampled.
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If a wound is large, collect sample from at least 1sqcm area both from wound bed and wound margins.
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The sample is sent to the laboratory immediately and is processed within 4 hrs of collection.
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The report is checked.
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Journal of Postgraduate Gynecology & Obstetrics is an Open Access, peer reviewed online journal published by Department of Obstetrics and Gynecology of Seth G. S. Medical College and K.E.M. Hospital, Parel, Mumbai, 400012, India.
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